Fission yeast spindle dynamics and chromosome segregation fidelity show distinct thermosensitivity

MicroPubl Biol. 2024 Jan 9:2024:10.17912/micropub.biology.001048. doi: 10.17912/micropub.biology.001048. eCollection 2024.

Abstract

Cellular processes rely on proteins with temperature-dependent stability and activity. While thermosensitivity in biological networks is well-explored, the effect of temperature on complex mechanochemical assemblies, like the spindle, is rarely studied. We examined fission yeast spindle dynamics and chromosome segregation from 15⁰C to 40⁰C. Our findings reveal that these parameters follow U-shaped temperature-dependent curves but reach their minima at different temperatures. Specifically, spindle dynamics peak around 35⁰C, whereas chromosome segregation defects are minimized at 25⁰C. This suggests a scenario in which mitotic errors are tolerated to expedite rapid cell cycle progression.

Grants and funding

This work was supported by grants from Fondation ARC and La Ligue Contre Le Cancer.