Flow velocity and light intensity combination is important for Microcystis aeruginosa physical suppression

Abstract

The cyanobacterial response to flow velocity or light intensity deviates from the combined effect of both factors. The responses of Microcystis aeruginosa to different combinations of flow velocities and light intensities were tested. Growth (OD₇₃₀ and protein), stress (catalase, ascorbate peroxidase, and glutathione peroxidase), and photosynthetic ability (chlorophyll-a and fluorescence) parameters of M. aeruginosa were measured to evaluate the effects of different combinations. Exposure to different flow velocity-light combinations significantly affected the growth and physiology of M. aeruginosa. Flow velocities of 0.4 m s^-1 showed a prominent influence on most of the measured parameters compared with no flow velocity or higher flow velocity conditions. The 1.2-m s^-1 flow velocity and high light intensity (1200 μmol m^-2 s^-1 ) exposure caused a significant elevation in oxidative stress. Lower velocities are beneficial for M. aeruginosa at light stress, whereas extreme velocities are adverse and elevate the stress. Two categories of light-velocity combinations were identified as preferred and extreme categories, depending on whether they suppressed or supported M. aeruginosa growth. In controlling cyanobacteria blooms using flow or high-intensity light, it is imperative to consider the interaction of these two factors, as their combined effects can significantly vary the stress levels in cyanobacteria. A new system, designed to minimize mechanical damage on M. aeruginosa, was used to generate flow velocities. Additionally, the combined effects of flow velocities and light intensities have been considered for the first time. PRACTITIONER POINTS: Flow velocity can influence the effect of light on Microcystis aeruginosa. High light exposure effect on Microcystis aeruginosa can be reduced by low flow velocity. High flow velocity and high light exposure increase the stress on Microcystis aeruginosa. Different light intensities and flow velocity combinations changed Microcystis aeruginosa stress physiology.

Keywords: antioxidant capacity; critical velocity; cyanobacteria bloom control; flow velocity simulation; oxidative stress.