Spermidine improves the antioxidant capacity and morphology of intestinal tissues and regulates intestinal microorganisms in Sichuan white geese

Zelong Wang; Dongmei Jiang; Xin Wang; Yilong Jiang; Qian Sun; Weikang Ling; Xiaoguang An; Chengweng Ji; Shuo Li; Yuxin Qi; Bo Kang

doi:10.3389/fmicb.2023.1292984

Spermidine improves the antioxidant capacity and morphology of intestinal tissues and regulates intestinal microorganisms in Sichuan white geese

Front Microbiol. 2024 Jan 16:14:1292984. doi: 10.3389/fmicb.2023.1292984. eCollection 2023.

Authors

Zelong Wang^#¹, Dongmei Jiang^#¹, Xin Wang^#¹, Yilong Jiang¹, Qian Sun¹, Weikang Ling¹, Xiaoguang An¹, Chengweng Ji¹, Shuo Li¹, Yuxin Qi¹, Bo Kang¹

Affiliation

¹ State Key Laboratory of Swine and Poultry Breeding Industry, Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.

^# Contributed equally.

Abstract

Introduction: Intestinal health is very important to the health of livestock and poultry, and is even a major determining factor in the performance of livestock and poultry production. Spermidine is a type of polyamine that is commonly found in a variety of foods, and can resist oxidative stress, promote cell proliferation and regulate intestinal flora.

Methods: In this study, we explored the effects of spermidine on intestinal health under physiological states or oxidative stress conditions by irrigation with spermidine and intraperitoneal injection of 3-Nitropropionic acid (3-NPA) in Sichuan white goose.

Results and discussion: Our results showed that spermidine could increase the ratio of intestinal villus to crypt and improve intestinal morphology. In addition, spermidine can also reduce malondialdehyde (MDA) accumulation caused by 3-NPA by increasing superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) enzyme activity, thus alleviating intestinal damage. Furthermore, spermidine can regulate intestinal digestive enzyme activities and affect intestinal digestion and absorption ability. Spermidine can also promote an increase in intestinal microbial diversity and abundance and alleviate the change of microflora structure caused by 3-NPA. In conclusion, spermidine promotes the production of beneficial intestinal metabolites such as Wikstromol, Alpha-bisabolol and AS 1-5, thus improving the level of intestinal health. Taken together, these results indicate that spermidine can improve intestinal health by improving intestinal morphology, increasing antioxidant capacity and regulating intestinal flora structure.

Keywords: antioxidant capacity; intestinal health; metabolomics; microorganisms; spermidine.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Natural Science Foundation of China (32172727, 31872358, and 31702116).