Antioxidant activity of spirostanol saponins from Allii Macrostemonis Bulbus and their contents in different origins and processed products

Jianfa Wu; Ying Cui; Chang Liu; Weixing Ding; Shen Ren; Jing Zhang; Lulu Wang

doi:10.1016/j.fochx.2024.101144

Antioxidant activity of spirostanol saponins from Allii Macrostemonis Bulbus and their contents in different origins and processed products

Food Chem X. 2024 Jan 17:21:101144. doi: 10.1016/j.fochx.2024.101144. eCollection 2024 Mar 30.

Authors

Jianfa Wu¹, Ying Cui¹, Chang Liu¹, Weixing Ding¹, Shen Ren^{1

2}, Jing Zhang^{1

2}, Lulu Wang³

Affiliations

¹ Department of Traditional Chinese Medicine, College of Traditional Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
² Jilin Provincial International Joint Research Center for the Development and Utilization of Authentic Medicinal Materials, Changchun 130118, China.
³ School of Medicine, Changchun Sci-Tech University, Changchun 130600, China.

Abstract

Allii Macrostemonis Bulbus (AMB), a traditional Chinese edible and medicinal plant, is considered beneficial to health. In this study, we isolated and purified nine steroidal saponins (compounds 1-9) from AMB. Their structures were characterized using physicochemical properties, HR-ESI-MS, 1D and 2D NMR spectroscopy. Among these compounds, compounds 1-5 were newly discovered named macrostemonoside U-Y, respectively. We assessed the in vitro antioxidant properties of the nine steroidal saponins through free radical scavenging and reducing power assays. This provides options for developing natural antioxidants. Additionally, an HPLC-ELSD quantitative analysis method was developed for the nine saponins in 12 batches of AMB from different origins and processing methods. The results showed that the contents of the nine steroidal saponins in AMB varied greatly among different growing environments and processing methods.

Keywords: Allii Macrostemonis Bulbus; Antioxidant activity; Content determination; Processing; Steroidal saponin.