A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia

Y Goa; J G Du; C Jirapattharasate; E Galon; S W Ji; Z G Ran; Y Q Xia

doi:10.47665/tb.40.4.004

A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia

Trop Biomed. 2023 Dec 1;40(4):400-405. doi: 10.47665/tb.40.4.004.

Authors

Y Goa^{1

2}, J G Du³, C Jirapattharasate⁴, E Galon⁵, S W Ji⁵, Z G Ran², Y Q Xia¹

Affiliations

¹ The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, 400715, PR China.
² Chongqing Auleon Biological Co., Ltd., Rongchang, Chongqing, 402460, PR China.
³ Department of Bacterial Biologics, China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Beijing 100-081, China.
⁴ Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, 999 Phutthamonthon Sai4, Salaya, Phutthamonthon, Nakhon Pathom, Thailand, 73170.
⁵ National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

PMID: 38308826
DOI: 10.47665/tb.40.4.004

Abstract

Beta toxin (CPB) is a lethal toxin and plays a key role in enterotoxemia of ruminants caused by Clostridium perfringens type C strain. The existing vaccines based on crude CPB need time-consuming detoxification and difficult quality control steps. In this study, we synthesized the rCPB_m4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPB_m4 (rSUMO-CPB_m4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPB_m4, rSUMO-CPB_m4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPB_m4 nor rSUMO-CPB_m4 was lethal to mice. Although rCPB_m4 and rSUMO-CPB_m4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPB_m4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge. These data suggest that genetically detoxified rSUMO-CPB_m4 is a promising subunit vaccine candidate for C. perfringens type C beta enterotoxemia.

MeSH terms

Animals
Bacterial Toxins* / genetics
Bacterial Vaccines
Clostridium Infections* / prevention & control
Clostridium Infections* / veterinary
Clostridium perfringens / genetics
Enterotoxemia / prevention & control
Mice
Rabbits
Recombinant Proteins / genetics

Substances

Bacterial Toxins
Bacterial Vaccines
Recombinant Proteins