Inheritance of genomic regions and genes associated with number of oocytes and embryos in Gir cattle through daughter design

R F B Rocha; A O Garcia; M G Dos Santos; P I Otto; M V B da Silva; M F Martins; M A Machado; J C C Panetto; M P L Calus; S E F Guimarães

doi:10.3168/jds.2023-24111

Inheritance of genomic regions and genes associated with number of oocytes and embryos in Gir cattle through daughter design

J Dairy Sci. 2024 Feb 2:S0022-0302(24)00060-2. doi: 10.3168/jds.2023-24111. Online ahead of print.

Authors

R F B Rocha¹, A O Garcia², M G Dos Santos², P I Otto³, M V B da Silva⁴, M F Martins⁴, M A Machado⁴, J C C Panetto⁴, M P L Calus⁵, S E F Guimarães⁶

Affiliations

¹ Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil; Animal Breeding and Genomics, Wageningen University & Research, 6700 AH Wageningen, the Netherlands.
² Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil.
³ Department of Animal Science, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
⁴ EMBRAPA - Dairy Cattle, Juiz de Fora, MG, 36038-330, Brazil.
⁵ Animal Breeding and Genomics, Wageningen University & Research, 6700 AH Wageningen, the Netherlands.
⁶ Department of Animal Science, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil. Electronic address: sfacioni@ufv.br.

PMID: 38310969
DOI: 10.3168/jds.2023-24111

Abstract

Over the past decades, daughter designs, including genotyped sires and their genotyped daughters, have been used as an approach to identify quantitative trait loci (QTL) related with economic traits. The aim of this study was to identify genomic regions inherited by Gir sire families and genes associated with number of viable oocytes (VO), total number of oocytes (TO) and number of embryos (EMBR) based on a daughter design approach. In total, 15 Gir sire families were selected. The number of daughters per family ranged from 26 to 395, which were genotyped with different SNP panels and imputed to the Illumina BovineHD BeadChip (777K) and had phenotypes for oocyte and embryo production. Daughters had phenotypic data for VO, TO, and EMBR. The search for QTL was performed through Genome-Wide Association Study (GWAS) based on genomic best linear unbiased prediction (gBLUP). QTL were found for each trait among and within families based on the top 10 genomic windows with the greatest genetic variance. For EMBR, genomic windows identified among families were located on BTA4, BTA5, BTA6, BTA7, BTA8, BTA13, BTA16 and BTA17, and they were most frequent on BTA7 within families. For VO, genomic windows were located on BTA2, BTA4, BTA5, BTA7, BTA17, BTA21, BTA22, BTA23 and BTA27 among families, being most frequent on BTA8 within families. For TO, top 10 genomic windows were identified on BTA2, BTA4, BTA5, BTA7, BTA17, BTA21, BTA22, BTA26 and BTA27, being most frequent on BTA7 and BTA8 within families. Considering all results, the greatest number of genomic windows was found on BTA7, where VCAN, XRCC4, TRNAC-ACA, HAPLN1 and EDIL3 genes were identified in the common regions. In conclusion, 15 Gir sire families with 26 to 395 daughters per family with phenotypes for oocyte and embryo production helped to identify the inheritance of several genomic regions, especially on BTA7, where EDIL3, HAPLN1 and VCAN candidate genes were associated with number of oocytes and embryos in Gir cattle families.

Keywords: bovine; family analysis; reproductive traits; zebu dairy cattle.

The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).