Effects of Benzo (a) Pyrene and 2,2',4,4'-Tetrabromodiphenyl Ether Exposure on the Thyroid Gland in Rats by Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy

QiuFeng Lao; LiJun Yang; ShuZhen Liu; XiaoJun Ma; DeChan Tan; JinBo Li; BaoYi Liao; YuanFeng Wei; WeiYi Pang; Camilo L M Morais; Hui Liu

doi:10.1021/acsomega.3c05819

Effects of Benzo (a) Pyrene and 2,2',4,4'-Tetrabromodiphenyl Ether Exposure on the Thyroid Gland in Rats by Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy

ACS Omega. 2024 Jan 19;9(4):4317-4323. doi: 10.1021/acsomega.3c05819. eCollection 2024 Jan 30.

Authors

QiuFeng Lao^{1

2

3}, LiJun Yang^{1

2}, ShuZhen Liu^{1

2}, XiaoJun Ma^{1

2}, DeChan Tan^{1

2}, JinBo Li^{1

2}, BaoYi Liao^{1

2}, YuanFeng Wei^{1

2}, WeiYi Pang¹, Camilo L M Morais⁴, Hui Liu^{1

2}

Affiliations

¹ Guangxi Key Laboratory of Environmental Exposomics and Entire Lifecycle Heath, Guilin Medical University, Guilin, Guangxi 541199, China.
² School of Public Health, Guilin Medical University, Guilin, Guangxi 541199, China.
³ Liuzhou People's Hospital, Liuzhou, Guangxi 545006, China.
⁴ Center for Education, Science and Technology of the Inhamuns Region, State University of Ceará, Tauá 63660-000, Brazil.

Abstract

Benzo[a]pyrene (B[a]P) and 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) are widespread environmental pollutants and can destroy thyroid function. We assessed the biochemical changes in the thyroid tissue of rats exposed to B[a]P and BDE-47 using attenuated total reflection Fourier-transform infrared spectroscopy combined with support vector machine(SVM). After B[a]P and BDE-47 treatment in rats, the structure of thyroid follicles was destroyed and epithelial cells were necrotic, indicating that B[a]P and BDE-47 may lead to changes of the thyroid morphology of the rats. These damages are mainly related to C=O stretch vibrations of lipids (1743 cm^-1), as well as the secondary structure of proteins [amide I (1645 cm^-1) and amide II (1550 cm^-1)], and carbohydrates [C-OH (1138 cm^-1), C-O (1106 cm^-1, 1049 cm^-1, 991 cm^-1), C-C (1106 cm^-1) stretching] and collagen (phosphodiester stretching at 922 cm^-1) vibration modes. When SVM was used for classification, there was a substantial separation between the control and the exposure groups (accuracy = 96%; sensitivity = 98%; specificity = 87%), and there was also a major separation between the exposed groups (accuracy = 93%; sensitivity = 94%; and specificity = 92%).