Secretory Production of the Hericium erinaceus Laccase from Saccharomyces cerevisiae

J Microbiol Biotechnol. 2024 Apr 28;34(4):930-939. doi: 10.4014/jmb.2312.12043. Epub 2024 Jan 22.


Mushroom laccases play a crucial role in lignin depolymerization, one of the most critical challenges in lignin utilization. Importantly, laccases can utilize a wide range of substrates, such as toxicants and antibiotics. This study isolated a novel laccase, named HeLac4c, from endophytic white-rot fungi Hericium erinaceus mushrooms. The cDNAs for this enzyme were 1569 bp in length and encoded a protein of 523 amino acids, including a 20 amino-acid signal peptide. Active extracellular production of glycosylated laccases from Saccharomyces cerevisiae was successfully achieved by selecting an optimal translational fusion partner. We observed that 5 and 10 mM Ca2+, Zn2+, and K+ increased laccase activity, whereas 5 mM Fe2+ and Al3+ inhibited laccase activity. The laccase activity was inhibited by the addition of low concentrations of sodium azide and L-cysteine. The optimal pH for the 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt was 4.4. Guaiacylglycerol-β-guaiacyl ether, a lignin model compound, was polymerized by the HeLac4c enzyme. These results indicated that HeLac4c is a novel oxidase biocatalyst for the bioconversion of lignin into value-added products for environmental biotechnological applications.

Keywords: Hericium erinaceus; Recombinant protein; Saccharomyces cerevisiae; laccase; secretion.

MeSH terms

  • Agaricales / enzymology
  • Agaricales / genetics
  • Amino Acid Sequence
  • Cloning, Molecular
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Glycosylation
  • Hericium* / enzymology
  • Hericium* / genetics
  • Hericium* / metabolism
  • Hydrogen-Ion Concentration
  • Laccase* / chemistry
  • Laccase* / genetics
  • Laccase* / metabolism
  • Lignin* / metabolism
  • Saccharomyces cerevisiae* / enzymology
  • Saccharomyces cerevisiae* / genetics
  • Saccharomyces cerevisiae* / metabolism
  • Sodium Azide / pharmacology


  • Laccase
  • Lignin
  • Fungal Proteins
  • Sodium Azide