Altered mitochondrial respiration in peripheral blood mononuclear cells of post-acute sequelae of SARS-CoV-2 infection

Sahera Dirajlal-Fargo; David P Maison; Jared C Durieux; Anastasia Andrukhiv; Nicholas Funderburg; Kate Ailstock; Mariana Gerschenson; Grace A Mccomsey

doi:10.1016/j.mito.2024.101849

Altered mitochondrial respiration in peripheral blood mononuclear cells of post-acute sequelae of SARS-CoV-2 infection

Mitochondrion. 2024 Mar:75:101849. doi: 10.1016/j.mito.2024.101849. Epub 2024 Feb 9.

Authors

Sahera Dirajlal-Fargo¹, David P Maison², Jared C Durieux³, Anastasia Andrukhiv⁴, Nicholas Funderburg⁵, Kate Ailstock⁶, Mariana Gerschenson⁷, Grace A Mccomsey⁸

Affiliations

¹ Case Western Reserve University, Cleveland, OH, USA; Ann and Robert Lurie Children's Hospital, Chicago, IL, USA. Electronic address: Sahera.dirajlal-fargo@uhhospitals.org.
² Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA. Electronic address: davidm22@hawaii.edu.
³ Case Western Reserve University, Cleveland, OH, USA. Electronic address: Jared.Durieux@UHhospitals.org.
⁴ Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA. Electronic address: andru78@hawaii.eduNicholas.
⁵ Division of Medical Laboratory Science, School of Health and Rehabilitation Sciences, The Ohio State University, Columbus, OH, USA. Electronic address: Nicholas.Funderburg@osumc.edu.
⁶ Division of Medical Laboratory Science, School of Health and Rehabilitation Sciences, The Ohio State University, Columbus, OH, USA. Electronic address: Kate.Ailstock@osumc.edu.
⁷ Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA. Electronic address: gerschen@hawaii.edu.
⁸ Case Western Reserve University, Cleveland, OH, USA. Electronic address: grace.mccomsey@uhhospitals.org.

PMID: 38341012
DOI: 10.1016/j.mito.2024.101849

Abstract

Peripheral blood mononuclear cells (PBMC) mitochondrial respiration was measured ex vivo from participants without a history of COVID (n = 19), with a history of COVID and full recovery (n = 20), and with PASC (n = 20). Mean mitochondrial basal respiration, ATP-linked respiration, maximal respiration, spare respiration capacity, ATP-linked respiration, and non-mitochondrial respiration were highest in COVID + PASC+ (p ≤ 0.04). Every unit increase in non-mitochondrial respiration, ATP-linked respiration, basal respiration, spare respiration capacity, and maximal respiration increased the predicted odds of PASC between 1 % and 6 %. Mitochondrial dysfunction in PBMCs may be contributing to the etiology of PASC.

Keywords: COVID; Long COVID; Mitochondria; PASC.

MeSH terms

Adenosine Triphosphate
COVID-19*
Disease Progression
Humans
Leukocytes, Mononuclear*
Post-Acute COVID-19 Syndrome
Respiration
SARS-CoV-2

Substances

Adenosine Triphosphate