Serotyping of dengue viruses by an enzyme-linked immunosorbent assay

Jpn J Med Sci Biol. Oct-Dec 1985;38(5-6):217-21. doi: 10.7883/yoken1952.38.217.

Abstract

We have developed a sandwich enzyme-linked immunosorbent assay for serotyping dengue viruses. In this assay, we used antibody from dengue hemorrhagic fever patients for detection of flavivirus common antigens to confirm virus isolation in C6/36 cells and that from hyperimmune mouse ascitic fluids for serotyping. The anti-dengue antibody was immobilized on microplate wells for capturing of dengue antigens, which were then sandwiched with the same biotinylated antibody. Then the biotin in the solid phase was detected with peroxidase-conjugated streptavidin. We found that all the dengue strains tested were unequivocally identified by this method.

MeSH terms

  • Antibodies, Viral
  • Antigen-Antibody Complex
  • Antigens, Viral / analysis
  • Biotin
  • Dengue / immunology
  • Dengue Virus / classification*
  • Dengue Virus / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Serotyping

Substances

  • Antibodies, Viral
  • Antigen-Antibody Complex
  • Antigens, Viral
  • Biotin