GABA synthesizing lactic acid bacteria and genomic analysis of Levilactobacillus brevis LAB6

Tushar Matta; Ruchika Bhatia; Santa Ram Joshi; Mahendra Bishnoi; Kanwaljit Chopra; Kanthi Kiran Kondepudi

doi:10.1007/s13205-024-03918-7

GABA synthesizing lactic acid bacteria and genomic analysis of Levilactobacillus brevis LAB6

3 Biotech. 2024 Mar;14(3):62. doi: 10.1007/s13205-024-03918-7. Epub 2024 Feb 7.

Authors

Tushar Matta^{1

2}, Ruchika Bhatia^{1

3}, Santa Ram Joshi⁴, Mahendra Bishnoi^{1

3

5}, Kanwaljit Chopra², Kanthi Kiran Kondepudi^{1

3

5}

Affiliations

¹ Healthy Gut Research Group, Centre of Excellence in Functional Foods, Food and Nutrition Biotechnology Laboratory, National Agri-Food Biotechnology Institute, S. A. S. Nagar, Mohali, Punjab, 140306 India.
² Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, 160014 India.
³ Department of Biotechnology, Panjab University, Chandigarh, India.
⁴ Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya 793022 India.
⁵ Regional Centre of Biotechnology, Faridabad, India.

PMID: 38344283
PMCID: PMC10850046 (available on 2025-03-01)
DOI: 10.1007/s13205-024-03918-7

Abstract

This study was conducted to investigate the γ-aminobutyric acid (GABA) production ability of 20 Lactobacillus and 25 Bifidobacterium strains which were previously isolated in our laboratory. Effect of initial pH, incubation time, monosodium glutamate (MSG), and pyridoxal-5'-phosphate (PLP) concentration for highest GABA production by two potent bacterial strains, Levilactobacillus brevis LAB6 and Limosilactobacillus fermentum LAB19 were optimized in the MRS media. A threefold increase in GABA production at an initial pH 4.0, incubation time of 120 h in medium supplemented with 3% MSG and 400 μM of PLP for LAB6 and 300 μM for LAB19 lead to the production of 19.67 ± 0.28 and 20.77 ± 0.14 g/L of GABA, respectively. Coculturing both strains under optimized conditions led to a GABA yield of 20.02 ± 0.17 g/L. Owing to potent anti-inflammatory activity in-vitro, as reported previously, and highest GABA production ability of LAB6 (MTCC 25662), its whole-genome sequencing and bioinformatics analysis was carried out for mining genes related to GABA metabolism. LAB6 harbored a complete glutamate decarboxylase (GAD) gene system comprising gadA, gadB, and gadC as well as genes responsible for the beneficial probiotic traits, such as for acid and bile tolerance and host adhesion. Comparative genomic analysis of LAB6 with 28 completely sequenced Levilactobacillus brevis strains revealed the presence of 95 strain-specific genes-families that was significantly higher than most other L. brevis strains.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-03918-7.

Keywords: Genome sequencing; Glutamate decarboxylase; Levilactobacillus brevis; γ-Aminobutyric acid (GABA).

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