In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and archiving of such structures. This report describes the workshop's motivation and history, the topics discussed, and the resulting consensus recommendations. Some challenges for future methods-development efforts in this area are also highlighted, as is the implementation to date of some of the recommendations.
Keywords: Electron Microscopy Data Bank; Protein Data Bank; bioinformatics; cryogenic-specimen electron microscopy; data archiving; databases; quality control; single-particle cryoEM; structure determination; validation.
open access.