What's new in single-cell proteomics

Curr Opin Biotechnol. 2024 Apr:86:103077. doi: 10.1016/j.copbio.2024.103077. Epub 2024 Feb 14.

Abstract

In recent years, single-cell proteomics (SCP) has advanced significantly, enabling the analysis of thousands of proteins within single mammalian cells. This progress is driven by advances in experimental design, with maturing label-free and multiplexed methods, optimized sample preparation, and innovations in separation techniques, including ultra-low-flow nanoLC. These factors collectively contribute to improved sensitivity, throughput, and reproducibility. Cutting-edge mass spectrometry platforms and data acquisition approaches continue to play a critical role in enhancing data quality. Furthermore, the exploration of spatial proteomics with single-cell resolution offers significant promise for understanding cellular interactions, giving rise to various phenotypes. SCP has far-reaching applications in cancer research, biomarker discovery, and developmental biology. Here, we provide a critical review of recent advances in the field of SCP.

Publication types

  • Review
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Mammals
  • Mass Spectrometry / methods
  • Proteins*
  • Proteome
  • Proteomics* / methods
  • Reproducibility of Results

Substances

  • Proteins
  • Proteome