Evaluation of Oxidative and Nitrosative Stress Markers Related To Inflammation in The Cumulus Cells and Follicular Fluid of Women Undergoing Intracytoplasmic Sperm Injection: A Prospective Study

Hasnae Debbarh; Malak Jamil; Hasnae Jelloul; Amal Kabit; Mohamed Ennaji; Noureddine Louanjli; Rachida Cadi

doi:10.22074/ijfs.2023.559526.1342

Evaluation of Oxidative and Nitrosative Stress Markers Related To Inflammation in The Cumulus Cells and Follicular Fluid of Women Undergoing Intracytoplasmic Sperm Injection: A Prospective Study

Int J Fertil Steril. 2024 Feb 2;18(2):108-114. doi: 10.22074/ijfs.2023.559526.1342.

Authors

Hasnae Debbarh¹, Malak Jamil^{2

3}, Hasnae Jelloul⁴, Amal Kabit³, Mohamed Ennaji³, Noureddine Louanjli^{3

4}, Rachida Cadi²

Affiliations

¹ Department of Biology, Laboratory of Molecular Genetic Physiopathology and Biotechnology, Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Morocco. Email: debbarhhasnae2017@gmail.com.
² Department of Biology, Laboratory of Molecular Genetic Physiopathology and Biotechnology, Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Morocco.
³ In vitro Fertilization Center IRIFIV, Iris Clinic, Casablanca, Morocco.
⁴ Labomac In Vitro Fertilization Center and Clinical Laboratory Medicine, Casablanca, Morocco.

Abstract

Background: Oxidative/nitrosative stress in the oocyte microenvironment could have an impact on intracytoplasmic sperm injection (ICSI) outcomes. The presence of reactive oxygen species (ROS) and reactive nitrogen species (RNS) can stimulate the secretion of pro-inflammatory cytokines, leading to chronic inflammation and potentially affecting embryo as well as oocyte quality. This study aimed to examine the relationship of lipid peroxidation [measured by the malondialdehyde (MDA) assay] with protein carbonyl [measured by the 2,4 dinitrophenylhydrazine (DNPH) assay] levels in cumulus cells (CCs), as well as nitric oxide (NO), peroxynitrite (ONOO^-), and C-reactive protein (CRP) levels in follicular fluid (FF). The potential relationship of these levels with ICSI outcome was also evaluated.

Materials and methods: In this prospective study, 63 FF samples and their corresponding CCs were collected for ICSI procedures. Spectrophotometry was used to assess levels of DNPH, MDA, NO, and ONOO^-. CRP levels were evaluated using an immunoturbidimetric assay.

Results: The patients under 37 years with normal ovarian reserve had significantly lower levels of MDA, DNPH, NO, ONOO^-, and CRP compared to those over 37 years. Additionally, we observed higher levels of MDA, DNPH, NO, ONOO^-, and CRP in the group with an oocyte maturity rate of less than 60%. No significant difference was observed between the DNPH levels and factors such as infertility duration, embryo quality, pregnancy, or the number of retrieved oocytes. A higher level of MDA, NO, ONOO^-, and CRP was found to be significantly related to the lower number of retrieved oocytes, longer periods of infertility, poor embryo quality, and negative pregnancy outcomes.

Conclusion: Oxidative/nitrosative stress, linking to inflammation in the oocyte microenvironment, can be considered as a potentially useful biomarker for assessing the development and competence of oocytes and embryos and predicting ICSI outcomes.

Keywords: Cumulus Cells; Follicular Fluid; Maternal Age; Oxidative/nitrosative Stress; Pregnancy.