Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery

J Microbiol Biotechnol. 2024 Apr 28;34(4):757-764. doi: 10.4014/jmb.2311.11024. Epub 2024 Feb 19.


Despite considerable advancements achieved using next-generation sequencing technologies in exploring microbial diversity, several species of the gut microbiome remain unknown. In this transformative era, culturomics has risen to prominence as a pivotal approach in unveiling realms of microbial diversity that were previously deemed inaccessible. Utilizing innovative strategies to optimize growth and culture medium composition, scientists have successfully cultured hard-to-cultivate microbes. This progress has fostered the discovery and understanding of elusive microbial entities, highlighting their essential role in human health and disease paradigms. In this review, we emphasize the importance of culturomics research on the gut microbiome and provide new theories and insights for expanding microbial diversity via the optimization of cultivation conditions.

Keywords: Culturomics; cultivation conditions; cultivation techniques; gut microbiome; medium optimization.

Publication types

  • Review

MeSH terms

  • Bacteria* / classification
  • Bacteria* / genetics
  • Bacteria* / growth & development
  • Biodiversity
  • Culture Media / chemistry
  • Gastrointestinal Microbiome*
  • High-Throughput Nucleotide Sequencing
  • Humans


  • Culture Media