Molecular cloning of the six mRNA species of infectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping

J Virol. 1985 Feb;53(2):469-76. doi: 10.1128/JVI.53.2.469-476.1985.

Abstract

Plasmids carrying cDNA sequences to the mRNA species of infectious hematopoietic necrosis virus were constructed and cloned into Escherichia coli. Characterization of 21 cloned plasmids by hybridization to mRNA blots identified sets of plasmids with homology to each of the six viral mRNA species. R-loop mapping with these cDNA plasmids determined that the gene order on the infectious hematopoietic necrosis virus genome is (3')N-M1-M2-G-NV-L(5').

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cloning, Molecular*
  • DNA
  • Genes, Viral*
  • Microscopy, Electron
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Hybridization
  • Plasmids
  • RNA, Messenger / genetics
  • RNA, Viral / genetics*
  • Rhabdoviridae / genetics*
  • Salmonidae / microbiology

Substances

  • Nucleic Acid Heteroduplexes
  • RNA, Messenger
  • RNA, Viral
  • DNA