Chromatographic characterisation of the circulating neuropeptide Y immunoreactivity from patients with phaeochromocytoma

Regul Pept. 1985 Mar;10(2-3):91-7. doi: 10.1016/0167-0115(85)90003-5.


Samples of adrenal medullary phaeochromocytoma from two patients were extracted in acid. The tissue concentrations of neuropeptide Y (NPY)-like immunoreactivity were found to be 0.94 and 8.11 micrograms/g. Plasma from these patients subjected to NPY radioimmunoassay after Sephadex G-75 gel filtration chromatography revealed circulating plasma concentrations of 1889 and 2079 pg/ml, compared to levels in normal plasma of less than 50 pg/ml, treated in the same way. Further characterisation of this plasma NPY-like immunoreactivity by HPLC showed this peptide to possess identical elution behaviour to human NPY standard. It is suggested that circulating NPY may be a contributory factor in the symptoms exhibited by some patients with adrenal medullary phaeochromocytoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Gland Neoplasms / analysis
  • Adrenal Gland Neoplasms / blood*
  • Chromatography, Gel
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / blood*
  • Neuropeptide Y
  • Pheochromocytoma / analysis
  • Pheochromocytoma / blood*
  • Radioimmunoassay


  • Nerve Tissue Proteins
  • Neuropeptide Y