Neuromedin U-8 and U-25: novel uterus stimulating and hypertensive peptides identified in porcine spinal cord

Biochem Biophys Res Commun. 1985 Aug 15;130(3):1078-85. doi: 10.1016/0006-291x(85)91726-7.


Two novel peptides eliciting a potent stimulant effect on the rat uterus smooth muscle have been purified and identified in porcine spinal cord. These peptides were designated as neuromedin U-8 (8 amino acids long) and U-25 (25 amino acids long) referring to their uterus stimulating activity. Sequence analyses and syntheses revealed that neuromedin U-8 is a novel octapeptide with a C-terminal amide structure, while U-25 contains the U-8 sequence at its C-terminus, preceded by paired Arg residues, implicating their biosynthetic relationship. Their potent uterus stimulating activity and hypertensive effect, as well as their unique C-terminal amide structure are indicative of their specialized physiological function.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Biological Assay
  • Blood Pressure / drug effects*
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Female
  • Hypertension / chemically induced
  • Nerve Tissue Proteins / isolation & purification*
  • Nerve Tissue Proteins / pharmacology
  • Neuropeptides*
  • Rats
  • Spinal Cord / physiology*
  • Structure-Activity Relationship
  • Swine
  • Uterine Contraction / drug effects*


  • Amino Acids
  • Nerve Tissue Proteins
  • Neuropeptides
  • neuromedin U 8
  • neuromedin U 25