Improved Viability of Spray-Dried Pantoea agglomerans for Phage-Carrier Mediated Control of Fire Blight

Viruses. 2024 Feb 6;16(2):257. doi: 10.3390/v16020257.

Abstract

Fire blight, caused by Erwinia amylovora, is a devastating bacterial disease that threatens apple and pear production. It is mainly controlled by using antibiotics, such as streptomycin. Due to development of E. amylovora resistant strains and the excessive agricultural use of antibiotics, there is an increased awareness of the possibility of antibiotic resistance gene transfer to other microbes. Urgent development of biocontrol agents (BCAs) is needed that can be incorporated into integrated pest management programs as antibiotic alternatives. A novel phage-carrier system (PCS) that combines an antagonistic bacterium, Pantoea agglomerans, with its ability to act as a phage-carrier bacterium for Erwinia phages has been developed. The low viability of P. agglomerans cells following spray-drying (SD) has been a challenge for the industrial-scale production of this PCS. Here, an SD protocol was developed for P. agglomerans by modifying the growth medium and bacterial cell formulation using D(+)-trehalose and maltodextrin. The developed protocol is amenable to the industrial-scale production of the BCA/PCS. The P. agglomerans viability was greater than 90% after SD and had a shelf life at 4 °C of 4 months, and reconstituted cells showed a 3 log reduction in E. amylovora counts with a pear disc assay.

Keywords: Erwinia amylovora; antibiotic resistance; apple; biological control agent; pear; streptomycin.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacteriophages* / genetics
  • Erwinia amylovora* / genetics
  • Malus*
  • Pantoea*
  • Plant Diseases / microbiology
  • Plant Diseases / prevention & control

Substances

  • Anti-Bacterial Agents

Grants and funding

This research was funded by Agriculture and Agri-Food Canada through an A-base research grant (J-002617 and J-002861) to H.A. and A.S., as well as the Natural Sciences and Engineering Research Council of Canada (Grant No. 229971 to J.W.).