Granagard administration prolongs the survival of human mesenchymal stem cells transplanted into a mouse model of multiple sclerosis

Kati Frid; Areen Usmann; Tsipora Markovits-Pachter; Orli Binyamin; Panayota Petrou; Ibrahim Kassis; Dimitri Karussis; Ruth Gabizon

doi:10.1016/j.jneuroim.2024.578313

Granagard administration prolongs the survival of human mesenchymal stem cells transplanted into a mouse model of multiple sclerosis

J Neuroimmunol. 2024 Apr 15:389:578313. doi: 10.1016/j.jneuroim.2024.578313. Epub 2024 Feb 14.

Authors

Kati Frid¹, Areen Usmann¹, Tsipora Markovits-Pachter¹, Orli Binyamin¹, Panayota Petrou², Ibrahim Kassis², Dimitri Karussis³, Ruth Gabizon⁴

Affiliations

¹ Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah-Hebrew University Hospital, Israel; Medical School, The Hebrew University, Jerusalem, Israel.
² Unit of Neuroimmunology and Cell therapies and Multiple Sclerosis Center, Hadassah-Hebrew University Hospital, Israel.
³ Medical School, The Hebrew University, Jerusalem, Israel; Unit of Neuroimmunology and Cell therapies and Multiple Sclerosis Center, Hadassah-Hebrew University Hospital, Israel.
⁴ Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah-Hebrew University Hospital, Israel; Medical School, The Hebrew University, Jerusalem, Israel. Electronic address: gabizonr@hadassah.org.il.

PMID: 38401393
DOI: 10.1016/j.jneuroim.2024.578313

Abstract

The clinical effect of human Mesenchymal stem cells (hMSCs) transplanted into EAE mice/MS patients is short lived due to poor survival of the transplanted cells. Since Granagard, a nanoformulation of pomegranate seed oil, extended the presence of Neuronal Stem cells transplanted into CJD mice brains, we tested whether this safe food supplement can also elongate the survival of hMSCs transplanted into EAE mice. Indeed, pathological studies 60 days post transplantation identified human cells only in brains of Granagard treated mice, concomitant with increased clinical activity. We conclude that Granagard may prolong the activity of stem cell transplantation in neurological diseases.

Keywords: EAE; Granagard; MS; Mesenchymal; Stem cells.

MeSH terms

Animals
Brain / pathology
Encephalomyelitis, Autoimmune, Experimental* / pathology
Encephalomyelitis, Autoimmune, Experimental* / therapy
Humans
Immunologic Factors
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells* / pathology
Mesenchymal Stem Cells* / physiology
Mice
Multiple Sclerosis* / pathology
Multiple Sclerosis* / therapy
Stem Cell Transplantation

Substances

Immunologic Factors