Detection of multiple serovars of Chlamydia trachomatis in genital infections

J Infect Dis. 1985 Nov;152(5):985-9. doi: 10.1093/infdis/152.5.985.


Eight genital isolates of Chlamydia trachomatis that each contained two different serovars were identified by typing with serovar-specific monoclonal antibodies. Mixed infections were confirmed by using serovar-specific monoclonal antibodies in an indirect immunofluorescence assay. For some isolates, fluorescein/rhodamine double-label indirect immunofluorescence with mixtures of IgM and IgG monoclonal antibodies to Chlamydia provided direct visual confirmation of mixed infection. Two isolates contained serovars D and F, and two E and F; one isolate contained serovars Ba and E, one D and J, one I' and F, and one E and J. In a sample of 352 genital isolates of C. trachomatis consecutively typed by dot ELISA with monoclonal antibodies, seven (2%) demonstrated mixed serovars.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Cervix Uteri / microbiology
  • Chlamydia Infections / microbiology*
  • Chlamydia trachomatis / classification*
  • Chlamydia trachomatis / immunology
  • Chlamydia trachomatis / isolation & purification
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Homosexuality
  • Humans
  • Male
  • Rectal Diseases / microbiology*
  • Rectum / microbiology
  • Serotyping
  • Sexually Transmitted Diseases / microbiology*
  • Uterine Cervical Diseases / microbiology*


  • Antibodies, Bacterial
  • Antibodies, Monoclonal