Dataset on stroke infarct volume in rodents: A comparison of MRI and histological methods

Rebecca Z Weber; Davide Bernardoni; Nora H Rentsch; Beatriz Achón Buil; Stefanie Halliday; Mark-Aurel Augath; Daniel Razansky; Christian Tackenberg; Ruslan Rust

doi:10.1016/j.dib.2024.110188

Dataset on stroke infarct volume in rodents: A comparison of MRI and histological methods

Data Brief. 2024 Feb 15:53:110188. doi: 10.1016/j.dib.2024.110188. eCollection 2024 Apr.

Authors

Rebecca Z Weber^{1

2}, Davide Bernardoni³, Nora H Rentsch^{1

2}, Beatriz Achón Buil^{1

2}, Stefanie Halliday¹, Mark-Aurel Augath^{4

5}, Daniel Razansky^{4

5}, Christian Tackenberg^{1

2}, Ruslan Rust^{1

2

6

7}

Affiliations

¹ Institute for Regenerative Medicine, University of Zurich, Schlieren 8952, Switzerland.
² Neuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland.
³ Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.
⁴ Faculty of Medicine, Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, University of Zurich, Zurich 8052, Switzerland.
⁵ Department of Information Technology and Electrical Engineering, Institute for Biomedical Engineering, ETH Zurich, Zurich 8093, Switzerland.
⁶ Department of Physiology and Neuroscience, University of Southern California, Los Angeles, CA 90089, United States.
⁷ Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, 1501 San Pablo St., Los Angeles, CA 900893, United States.

Abstract

This dataset offers images of mouse brains impacted by photothrombotic stroke in the sensorimotor cortex published by Weber et al. NeuroImage (2024). Data is gathered using two primary techniques: (1) whole-brain ex-vivo magnetic resonance imaging (MRI) and (2) 40 µm thick coronal histological sections that undergo immunofluorescence staining with NeuroTrace. Infarct areas and volumes are assessed through MRI at two distinct time frames-three days (acute) and 28 days (chronic) following photothrombotic stroke induction. Subsequently, the brains are sectioned into 40 µm thick coronal slices, stained with NeuroTrace, and imaged as whole sections. The dataset holds considerable value for reuse, particularly for researchers focused on stroke volume estimation methods as well as those interested in comparing the efficacy of MRI and histological techniques.

Keywords: Histology; Ischemia; MRI; Preclinical stroke research; Stroke outcome; Stroke volume.