Molecular Forms of Glucagon-Like Peptides in Man

FEBS Lett. 1985 Nov 18;192(2):275-8. doi: 10.1016/0014-5793(85)80124-1.

Abstract

Molecular forms of the glucagon-like peptides (GLP) encoded by the human preproglucagon gene were analysed by chromatography combined with specific radioimmunoassays to the synthetic peptides. Whereas extracts of human pancreas and a glucagonoma contained a large proglucagon cleavage product possessing both GLP-1 and GLP-2 immunoreactivities, extracts of human intestine contained products corresponding to free GLP-1 and a small amount of chromatographically distinct GLP-2 immunoreactivity. It is concluded that post-translational processing of proglucagon differs in pancreas and intestine, so that the C-terminal portion of the molecule is cleaved to liberate free GLP-1 in the intestine. Further processing or degradation results in loss especially of GLP-2 immunoreactivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Genes*
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptide 2
  • Glucagonoma / metabolism
  • Humans
  • Intestinal Mucosa / metabolism
  • Pancreas / metabolism
  • Pancreatic Neoplasms / metabolism
  • Peptides / genetics*
  • Peptides / isolation & purification
  • Protein Precursors / genetics*
  • Radioimmunoassay

Substances

  • Glucagon-Like Peptide 2
  • Peptides
  • Protein Precursors
  • Glucagon-Like Peptide 1