Release and degradation of neurotensin during perfusion of rat small intestine with lipid

Regul Pept. 1985 Oct;12(2):101-11. doi: 10.1016/0167-0115(85)90191-0.

Abstract

The levels of neurotensin (NT) and its metabolite, the N-terminal octapeptide (NT1-8), identified by HPLC and measured by RIA, were increased in the hepatic-portal circulation of the anesthetized rat during perfusion of the small intestine with a lipid solution, while levels of both peptides remained unchanged in the general circulation. There was no significant arteriovenous difference for NT or NT1-8 during saline perfusion of the small intestine. Plasma collected from the superior mesenteric vein during the infusion of [3H]NT into the superior mesenteric artery showed major peaks of radioactivity with the retention times of NT1-8 and NT1-11 on HPLC. Only 12% of the radioactivity recovered from plasma was intact NT. These studies demonstrate that chromatographically identified NT and its metabolite, NT1-8, are elevated in the portal circulation but not systemic circulation during lipid perfusion and that the small intestine may be both the site of release and metabolism of NT.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Deoxycholic Acid / analogs & derivatives*
  • Female
  • Glycerides / pharmacology*
  • Intestine, Small / drug effects
  • Intestine, Small / metabolism*
  • Kinetics
  • Liver / metabolism
  • Male
  • Micelles
  • Neurotensin / metabolism*
  • Oleic Acid
  • Oleic Acids / pharmacology*
  • Peptide Fragments / metabolism
  • Perfusion
  • Pyrrolidonecarboxylic Acid / analogs & derivatives
  • Rats
  • Rats, Inbred Strains
  • Taurodeoxycholic Acid / pharmacology*
  • Tritium

Substances

  • Glycerides
  • Micelles
  • Oleic Acids
  • Peptide Fragments
  • Deoxycholic Acid
  • Tritium
  • Oleic Acid
  • Neurotensin
  • Taurodeoxycholic Acid
  • neurotensin (1-8)
  • monoolein
  • Pyrrolidonecarboxylic Acid