The nucleotide sequence of the env gene from the human provirus ERV3 and isolation and characterization of an ERV3-specific cDNA

Virology. 1985 Dec;147(2):449-58. doi: 10.1016/0042-6822(85)90147-3.

Abstract

The nucleotide sequence of the env gene of a previously described human provirus (ERV3) has been determined beginning near the 3'-end of the pol gene and continuing through the 3'-LTR. Analysis of the nucleotide sequence revealed the presence of a long open reading frame of 1944 nucleotides that is capable of encoding a polypeptide that has characteristics of other retroviral glycoproteins and transmembrane proteins. These include the presence of seven potential glycosylation sites, a typical glycoprotein-transmembrane protein cleavage sequence, and amino acid homologies to the glycoproteins and transmembrane proteins of other retroviruses. Further, we have isolated an ERV3-specific cDNA clone from a library prepared from liver RNA of a 20-week human fetus. DNA sequence analysis of this clone revealed that it is identical to the ERV3 genomic clone in the 1110 nucleotides that were sequenced.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Codon
  • DNA / genetics*
  • DNA / isolation & purification
  • Fetus
  • Genes, Viral*
  • Glycoproteins / genetics*
  • Humans
  • Retroviridae / genetics*
  • Viral Envelope Proteins / genetics*

Substances

  • Codon
  • Glycoproteins
  • Viral Envelope Proteins
  • DNA

Associated data

  • GENBANK/M12140