Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli

Mol Gen Genet. 1979 May 4;172(2):171-8. doi: 10.1007/BF00268279.

Abstract

Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Restriction Enzymes / metabolism
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics*
  • Genes*
  • Genetic Linkage
  • Nucleic Acid Hybridization
  • Plasmids*
  • RNA, Ribosomal / genetics*
  • Recombination, Genetic

Substances

  • DNA, Bacterial
  • RNA, Ribosomal
  • DNA Restriction Enzymes