Neuromedins: novel smooth-muscle stimulating peptides identified in porcine spinal cord

Peptides. 1985;6 Suppl 3:245-8. doi: 10.1016/0196-9781(85)90381-x.

Abstract

Two novel peptides, neuromedin U-8 and U-25, eliciting a potent uterus stimulating activity, have been purified and identified in porcine spinal cord. Sequence analyses and syntheses revealed that neuromedin U-8 is a novel octapeptide with a C-terminal amide structure, while U-25 contains the U-8 sequence at its C-terminus, preceded by paired Arg residues, implicating their biosynthetic relationship. Their potent uterus stimulating activity and hypertensive effect, as well as their unique C-terminal amide structure are indicative of their specialized physiological function. In addition, by utilizing a specific radioimmunoassay for neuromedin B that is a bombesin-like peptide identified in porcine spinal cord, we have isolated two novel "big" neuromedin B, designated neuromedin B-32 and B-30, from pig brain and spinal cord, indicative of their biosynthetic relationship. Nine neuromedins (B, B-30, B-32, C, K, L, N, U-8 and U-25) thus far identified in porcine spinal cord as the smooth-muscle stimulating peptides, are classified into four families; B-(bombesin-like), K-(kassinine-like), N-(neurotensin-like) and U-groups.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Nerve Tissue Proteins / isolation & purification*
  • Neurokinin B* / analogs & derivatives*
  • Neuropeptides*
  • Oligopeptides / isolation & purification*
  • Spinal Cord / analysis*
  • Swine / physiology

Substances

  • Nerve Tissue Proteins
  • Neuropeptides
  • Oligopeptides
  • Neurokinin B
  • neuromedin B
  • neuromedin U 8
  • neuromedin U 25