Objective: To investigate the changes of spinal vascular blood flow in SD rats after cervical, thoracic and lumbar spinal cord injury (SCI) using super-resolution ultrafast ultrasound technology. Methods: A total of 9 SD rats were used to construct SCI models at different segments using a 50 g aneurysm clip. Super-resolution ultrafast ultrasound technology was used to perform vascular blood flow imaging on the spinal cord of rats before and after injury at 6 hours, obtaining quantitative information such as spinal cord vascular density and blood flow velocity. Results: Ultrasound imaging showed that after SCI, the vascular density in the thoracic segment decreased (18.16%±1.04%) more than in the cervical segment (11.42%±1.39%) and lumbar segment (13.88%±1.43%, both P<0.05). The length of the spinal cord with decreased vascular density in the thoracic segment [(4.80±0.34)mm] was longer than that in the cervical segment [(2.80±0.57)mm] and lumbar segment [(3.10±0.36)mm, both P<0.05]. After injury, the decrease of blood flow in the thoracic segment [(8.87±0.85)ml/min] was higher than that in the cervical segment [(4.88±0.56)ml/min] and lumbar segment [(6.19±0.71)ml/min, both P<0.05]. HE staining and Nissl staining showed that the proportion of cavity area after thoracic SCI (11.53%±0.93%) was higher than that in the cervical segment (4.90%±1.72%) and lumbar segment (7.64%±0.84%, both P<0.05). The number of Nissl bodies in the thoracic segment (18.0±5.3) was also lower than that in the cervical segment (32.3±5.1) and lumbar segment (37.0±5.6) (both P<0.05). Conclusions: There are different changes in vascular blood flow after SCI in different segments of rats. The same injury causes the most severe damage to blood vessels in the thoracic spinal cord, followed by the lumbar spinal cord, and the cervical spinal cord has the least damage.
目的： 通过超分辨率超快超声技术观测颈、胸、腰不同节段脊髓损伤后SD大鼠脊髓血管血流的变化情况。 方法： 共9只SD大鼠使用50 g动脉瘤夹损伤脊髓来构建不同节段大鼠脊髓损伤模型，损伤前及损伤后6 h使用超分辨率超快超声技术对损伤前后的SD大鼠脊髓进行血管血流成像，得到脊髓血管密度、血流速度等定量信息。 结果： 超声成像显示，脊髓损伤后胸段血管密度下降幅度（18.16%±1.04%）多于颈段（11.42%±1.39%）与腰段（13.88%±1.43%）（均P<0.05）；胸段发生血管密度下降的脊髓长度［（4.80±0.34）mm］长于颈段［（2.80±0.57）mm］与腰段［（3.10±0.36）mm，均P<0.05］；损伤后胸段血流量下降［（8.87±0.85）ml/min］高于颈段［（4.88±0.56）ml/min］与腰段［（6.19±0.71）ml/min，均P<0.05］。HE染色与尼氏染色显示胸段脊髓损伤后空腔面积占比（11.53%±0.93%）高于颈段（4.90%±1.72%）和腰段（7.64%±0.84%）（均P<0.05），且胸段尼氏体数量（18.0±5.3）也少于颈段（32.3±5.1）与腰段（37.0±5.6）脊髓损伤（均P<0.05）。 结论： 不同节段SD大鼠脊髓损伤后血管血流改变存在不同，相同损伤在胸段脊髓对血管的破坏最严重，腰段脊髓次之，颈段脊髓损坏最小。.