The integration of complementary analytical platforms is nowadays the most common strategy for comprehensive metabolomics analysis of complex biological systems. In this chapter, we describe methods and tips for the application of a mass spectrometry multi-platform in Alzheimer's disease research, based on the combination of direct mass spectrometry and orthogonal hyphenated approaches, namely, reversed-phase ultrahigh-performance liquid chromatography and gas chromatography. These procedures have been optimized for the analysis of multiple biological samples from human patients and transgenic animal models, including blood serum, various brain regions (e.g., hippocampus, cortex, cerebellum, striatum, olfactory bulbs), and other peripheral organs (e.g., liver, kidney, spleen, thymus).
Keywords: Alzheimer’s disease; Direct MS analysis; Gas chromatography; Mass spectrometry; Metabolomics; Multi-platform; Ultrahigh-performance liquid chromatography.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.