Chemical Constituents, Hypolipidemic, and Hypoglycemic Activities of Edgeworthia gardneri Flowers

Xiaoyu Zhang; Qingwang Xue; Jinghao Zhao; Hongbin Zhang; Jiahong Dong; Jianxin Cao; Yudan Wang; Yaping Liu; Guiguang Cheng

doi:10.1007/s11130-024-01154-1

Chemical Constituents, Hypolipidemic, and Hypoglycemic Activities of Edgeworthia gardneri Flowers

Plant Foods Hum Nutr. 2024 Mar 5. doi: 10.1007/s11130-024-01154-1. Online ahead of print.

Authors

Xiaoyu Zhang¹, Qingwang Xue², Jinghao Zhao³, Hongbin Zhang¹, Jiahong Dong¹, Jianxin Cao¹, Yudan Wang³, Yaping Liu⁴, Guiguang Cheng⁵

Affiliations

¹ The Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China.
² Department of Chemistry, Liaocheng University, Liaocheng, 252059, China.
³ Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming, 650500, China.
⁴ The Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China. liuyaping@kmust.edu.cn.
⁵ The Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China. ggcheng@kmust.edu.cn.

PMID: 38441843
DOI: 10.1007/s11130-024-01154-1

Abstract

The flowers of Edgeworthia gardneri are used as herbal tea and medicine to treat various metabolic diseases including hyperglycemia, hypertension, and hyperlipidemia. This paper investigate the chemical constituents and biological activities of ethanolic extract and its different fractions from E. gardneri flowers. Firstly, the E. gardneri flowers was extracted by ethanol-aqueous solution to obtain crude extract (CE), which was subsequently fractionated by different polar organic solution to yield precipitated crystal (PC), dichloromethane (DCF), ethyl acetate (EAF), n-butanol (n-BuF), and residue water (RWF) fractions. UHPLC-ESI-HRMS/MS analysis resulted in the identification of 25 compounds, and the main compounds were flavonoids and coumarins. The precipitated crystal fraction showed the highest phenolic and flavonoid contents with 344.4 ± 3.38 mg GAE/g extract and 305.86 ± 0.87 mg RE/g extract. The EAF had the strongest antioxidant capacity and inhibitory effect on α-glucosidase and pancreatic lipase with IC₅₀ values of 126.459 ± 7.82 and 23.16 ± 0.79 µg/mL. Besides, both PC and EAF significantly regulated the glucose and lipid metabolism disorders by increasing glucose consumption and reducing TG levels in HepG2 cells. Molecular docking results suggested that kaempferol-3-O-glucoside and tiliroside had good binding ability with enzymes, indicating that they may be potential α-glucosidase and pancreatic lipase inhibitors. Therefore, the E. gardneri flowers could be served as a bioactive agent for the regulation of metabolic disorders.

Keywords: Edgeworthia gardneri; Glucose and lipid metabolism; Pancreatic lipase enzyme; UHPLC-ESI-HRMS/MS; α-glucosidase Enzyme.

Abstract

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