Genome-scale requirements for dynein-based transport revealed by a high-content arrayed CRISPR screen

J Cell Biol. 2024 May 6;223(5):e202306048. doi: 10.1083/jcb.202306048. Epub 2024 Mar 6.


The microtubule motor dynein plays a key role in cellular organization. However, little is known about how dynein's biosynthesis, assembly, and functional diversity are orchestrated. To address this issue, we have conducted an arrayed CRISPR loss-of-function screen in human cells using the distribution of dynein-tethered peroxisomes and early endosomes as readouts. From a genome-wide gRNA library, 195 validated hits were recovered and parsed into those impacting multiple dynein cargoes and those whose effects are restricted to a subset of cargoes. Clustering of high-dimensional phenotypic fingerprints revealed co-functional proteins involved in many cellular processes, including several candidate novel regulators of core dynein functions. Further analysis of one of these factors, the RNA-binding protein SUGP1, indicates that it promotes cargo trafficking by sustaining functional expression of the dynein activator LIS1. Our data represent a rich source of new hypotheses for investigating microtubule-based transport, as well as several other aspects of cellular organization captured by our high-content imaging.

MeSH terms

  • CRISPR-Cas Systems
  • Dyneins* / genetics
  • Genetic Techniques
  • Humans
  • Microtubules* / genetics
  • Peroxisomes / genetics


  • Dyneins