5-Methyl-2-thiouridine in the tRNA of Candida tropicalis and its localization in lysine tRNA

J Bacteriol. 1985 Apr;162(1):55-60. doi: 10.1128/jb.162.1.55-60.1985.

Abstract

35S incorporation studies showed that Candida tropicalis tRNA contained two thionucleosides, one of which was identified as 5-methyl-2-thiouridine. The other thionucleoside was alkali labile, and it appeared to be an ester. Pulse-chase experiments suggested that the two thionucleosides were structurally related. 5-Methyl-2-thiouridine was present in one of the lysine tRNAs. This is the first report of the presence of this nucleoside in a yeast tRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida / analysis*
  • RNA, Fungal / analysis*
  • RNA, Transfer / analysis*
  • RNA, Transfer, Amino Acyl / analysis*
  • Thionucleosides / analysis*
  • Thionucleosides / biosynthesis
  • Thiouridine / analogs & derivatives*
  • Thiouridine / analysis

Substances

  • RNA, Fungal
  • RNA, Transfer, Amino Acyl
  • Thionucleosides
  • Thiouridine
  • 2-thioribothymidine
  • RNA, Transfer