Study of chondrogenesis of umbilical cord mesenchymal stem cells in curdlan- poly(vinyl alcohol) composite hydrogels and its mechanical properties of freezing-thawing treatments

Yu-Chieh Su; Yu Chang; Wei-Chang Lee; Jhih-Han Wang; Takumi Narita; Hiroyuki Takeno; Jie-Yu Syu; I-Ming Jou; Wen-Chuan Hsieh

doi:10.1016/j.ijbiomac.2024.130792

Study of chondrogenesis of umbilical cord mesenchymal stem cells in curdlan- poly(vinyl alcohol) composite hydrogels and its mechanical properties of freezing-thawing treatments

Int J Biol Macromol. 2024 Apr;265(Pt 1):130792. doi: 10.1016/j.ijbiomac.2024.130792. Epub 2024 Mar 11.

Authors

Yu-Chieh Su¹, Yu Chang², Wei-Chang Lee³, Jhih-Han Wang⁴, Takumi Narita⁵, Hiroyuki Takeno⁶, Jie-Yu Syu⁴, I-Ming Jou⁷, Wen-Chuan Hsieh⁸

Affiliations

¹ Division of Hematology-Oncology, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan; School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan.
² Department of Obstetrics and Gynecology, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan; School of Medicine for International Students, College of Medicine, I-Shou University, Kaohsiung, Taiwan.
³ Division of Hematology-Oncology, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
⁴ Department of Medical Science and Biotechnology, I-Shou University, Kaohsiung 824005, Taiwan.
⁵ Division of Molecular Science, Graduate School of Science and Technology, Gunma University, Gunma 376-8515, Japan.
⁶ Division of Molecular Science, Graduate School of Science and Technology, Gunma University, Gunma 376-8515, Japan; Gunma University Center for Food Science and Wellness (GUCFW), Gunma 376-8515, Japan.
⁷ Department of Orthopedics, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
⁸ Department of Medical Science and Biotechnology, I-Shou University, Kaohsiung 824005, Taiwan. Electronic address: wenchuan@isu.edu.tw.

PMID: 38479670
DOI: 10.1016/j.ijbiomac.2024.130792

Abstract

The curdlan gel is a natural material produced by bacteria. It utilizes chemical cross-linking reactions to form a 3D porous composite hydrogel, increasing its porosity and water content, and improving its mechanical properties. It can be used in tissue repair and regenerative medicine. Curdlan-Poly(vinyl alcohol) (PVA) composite hydrogel can rapidly swell within 1 min due to its porous structure. Compression tests confirmed that it still maintains its original mechanical strength, even after five repeated freeze-thaw (FT) processes, making it suitable for long-term cryopreservation. The purpose of this study is to transplant umbilical cord mesenchymal stem cells (UC-MSCs) on Curdlan-PVA composite hydrogel and observe the chondrocytes on the material. The results of using 4',6-diamidino-2-phenylindole (DAPI), hematoxylin and eosin (H&E), calcein-acetoxymethyl ester (calcein AM), and Collagen type II-Fluorescein isothiocyanate (FITC) staining, confirmed that UC-MSCs can attach and differentiate into chondrocytes on 3D Curdlan-PVA composite hydrogel.

Keywords: Chondrocytes; Composite hydrogel; Curdlan; Umbilical cord mesenchymal stem cells.

MeSH terms

Biocompatible Materials / chemistry
Chondrogenesis
Ethanol
Freezing
Hydrogels* / chemistry
Hydrogels* / pharmacology
Mesenchymal Stem Cells*
Polyvinyl Alcohol / chemistry
beta-Glucans*

Substances

Hydrogels
Polyvinyl Alcohol
curdlan
Biocompatible Materials
Ethanol
beta-Glucans