Xylooligosaccharide supplementation in rice protein concentrate based diets: A comprehensive analysis of performance and health of Labeo rohita

Ayesha Khizar; Mahroze Fatima; Noor Khan; Muhammad Afzal Rashid

doi:10.1111/jpn.13951

Xylooligosaccharide supplementation in rice protein concentrate based diets: A comprehensive analysis of performance and health of Labeo rohita

J Anim Physiol Anim Nutr (Berl). 2024 Mar 18. doi: 10.1111/jpn.13951. Online ahead of print.

Authors

Ayesha Khizar¹, Mahroze Fatima¹, Noor Khan², Muhammad Afzal Rashid³

Affiliations

¹ Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan.
² Institute of Zoology, University of Punjab, Lahore, Pakistan.
³ Department of Animal Nutrition, University of Veterinary and Animal Sciences, Lahore, Pakistan.

PMID: 38500315
DOI: 10.1111/jpn.13951

Abstract

The primary aim of this study was to examine the impact of xylooligosaccharide (XOS) in rice protein concentrate (RPC) based diets on the growth performance, body composition, digestive enzymes, intestinal morphology and blood biochemistry of Labeo rohita fingerlings. Four different XOS levels (0%, 0.5%, 1% and 2%) were used at each RPC (75% and 100%) level. Twenty-five fish per tank with an average initial weight of 25 ± 0.05 g were randomly assigned (Randomised complete block design) to each of the 8 groups in triplicate aquaria (36 × 16 × 12″) and then fed with respective diets @ 3% body weight for 90 days. The results showed significant improvements in growth performance, such as increased weight gain %, specific growth rate, and protein efficiency ratio and improved feed conversion ratio in 1% XOS supplemented diet at 75% RPC. A significant decrease in serum alkaline phosphatase activity (ALP) and plasma melanodialdehyde (MDA) were observed at 1% XOS level in 75% RPC based diets, respectively. Meanwhile, the lowest total cholesterol and highest lysozyme activity were observed in 1% XOS supplemented diet at 75% RPC levels. Moreover, the serum (alanine aminotransferase and aspartate transaminase) and plasma (superoxide dismutase, triglyceride, high density and low density lipoprotein) activities showed nonsignificant effects among the treatments. Furthermore, the digestive enzymes (protease & lipase) and intestinal morphology were significantly influenced at 1% XOS in the 75% RPC-based diet. Polynomial regression analysis showed that 1.25% XOS is the optimum requirement for the growth of rohu fingerlings when fed at 75% RPC based diets. Overall, it was concluded that the 75% RPC diet was efficiently replaced by fishmeal along with 1% XOS addition in L. rohita fingerlings without any negative effect on growth performance and intestinal health.

Keywords: Growth performance; Rohu; digestive enzymes; immunity; intestinal health; plasma profile; rice protein concentrate; xylooligosaccharide.