Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP

Methods Mol Biol. 2024:2784:177-189. doi: 10.1007/978-1-0716-3766-1_12.

Abstract

Fluorescent in situ hybridization (FISH) enables the visualization of the position and abundance of nucleic acid molecules in fixed cell and tissue samples. Many FISH-based methods employ sets of synthetic, computationally designed DNA oligonucleotide (oligo) FISH probes, including massively multiplexed imaging spatial transcriptomics and spatial genomics technologies. Oligo probes can either be designed de novo or accessed from an existing database of pre-discovered probe sequences. This chapter describes the use of PaintSHOP, a user-friendly, web-based platform for the design of sets of oligo-based FISH probes. PaintSHOP hosts large collections of pre-discovered probes from many model organisms and also provides collections of functional sequences such as primers and readout domains and interactive tools to add these functional sequences to selected probes. Detailed examples are provided for three common experimental scenarios.

Keywords: Chromatin; Chromosomes; Cytogenetics; FISH; Oligonucleotide; Spatial genomics; Spatial transcriptomics.

MeSH terms

  • DNA Primers
  • Genomics*
  • In Situ Hybridization, Fluorescence / methods
  • Oligonucleotide Probes / genetics

Substances

  • Oligonucleotide Probes
  • DNA Primers