The effects of topical aloe vera on an experimentally designed penile fracture model in rats

Ulus Travma Acil Cerrahi Derg. 2024 Mar;30(3):147-154. doi: 10.14744/tjtes.2024.26425.


Background: This study assessed the histopathological and oxidative effects of topical Aloe Vera (AV) on penile fractures (PF) formed experimentally in a rat model.

Methods: Forty Wistar albino rats (220-250 g) were used. The PF model was created experimentally with a number 15 lancet. Then, the rats were randomly and equally divided into five groups. In the first group (C), no incision was formed. In the second group (P), an incision was formed. In the third group (PR), the incision line was closed primarily. In the fourth group (PA), AV was locally applied onto the incision without suturing for three days. In the last group (PRA), AV was applied to the primary repair region for three days. All groups were compared to each other according to histopathological and biochemical data.

Results: Hyperemia-bleeding was observed to be suppressed in the PRA group compared to the other groups (p<0.001). Inflammation was observed only in Groups PR and PRA (p<0.001). Significant fibrosis was observed in the PA and PRA groups compared to the other groups (p<0.001). Superoxide Dismutase (SOD) and Glutathione (GSH) values increased in favor of Group PRA (p=0.009 and p=0.035, respectively). Total Oxidative Status (TOS) and Malondialdehyde (MDA) values decreased in favor of Group PA (p=0.036 and p=0.026, respectively). Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-1 beta (IL-1ß) levels decreased mostly in the PRA group, but these changes did not reach statistical significance (p>0.05).

Conclusion: Topical AV application reduces tissue inflammation and oxidative stress but appears to increase the development of fibrosis after PF.

MeSH terms

  • Aloe* / metabolism
  • Animals
  • Fibrosis
  • Glutathione
  • Humans
  • Inflammation
  • Male
  • Malondialdehyde / pharmacology
  • Oxidative Stress
  • Penile Diseases*
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Superoxide Dismutase / pharmacology


  • Glutathione
  • Superoxide Dismutase
  • Malondialdehyde