A simplified version of rapid susceptibility testing of bacteria and yeasts using optical nanomotion detection

Front Microbiol. 2024 Mar 7:15:1328923. doi: 10.3389/fmicb.2024.1328923. eCollection 2024.


We present a novel optical nanomotion-based rapid antibiotic and antifungal susceptibility test. The technique consisted of studying the effects of antibiotics or antifungals on the nanometric scale displacements of bacteria or yeasts to assess their sensitivity or resistance to drugs. The technique relies on a traditional optical microscope, a video camera, and custom-made image analysis software. It provides reliable results in a time frame of 2-4 h and can be applied to motile, non-motile, fast, and slowly growing microorganisms. Due to its extreme simplicity and low cost, the technique can be easily implemented in laboratories and medical centers in developing countries.

Keywords: antimicrobials; bacteria; nanomotion; optical microscope; yeast.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the Belgian Federal Science Policy Office (Belspo) and the European Space Agency, grant number PRODEX project Flumias Nanomotion; The Research Foundation – Flanders (FWO), grant numbers AUGE/13/19 and I002620; FWO-SNSF, grant number 310030L_197946. VG and SR were supported by SNSF project grant 310030_204938 awarded to SR.