Profiling the Phytochemicals of Orostachys margaritifolia: Biological Activities, LC-ESI/MS, and HPLC Analyses

Nari Yoon; Hyeonjun Yu; Gia Han Tran; Chung Ho Ko; Hoon Kim; Mi Jeong Yoon; Sanghyun Lee

doi:10.3390/ph17030290

Profiling the Phytochemicals of Orostachys margaritifolia: Biological Activities, LC-ESI/MS, and HPLC Analyses

Pharmaceuticals (Basel). 2024 Feb 23;17(3):290. doi: 10.3390/ph17030290.

Authors

Nari Yoon¹, Hyeonjun Yu², Gia Han Tran¹, Chung Ho Ko³, Hoon Kim⁴, Mi Jeong Yoon³, Sanghyun Lee^{1

5}

Affiliations

¹ Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
² Department of Integrated Biomedical and Life Sciences, Korea University, Seoul 02841, Republic of Korea.
³ Division of Garden and Plant Resources, Korea National Arboretum, Pocheon 11186, Republic of Korea.
⁴ Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Republic of Korea.
⁵ Natural Product Institute of Science and Technology, Anseong 17546, Republic of Korea.

Abstract

Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS⁺ and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS⁺ and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC₅₀ value of 202.6 μg/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. The study revealed possibilities for the utilization of OMY as a future source of drugs for alleviating inflammation and diseases related to reactive oxygen species.

Keywords: ABTS+-DPPH radical scavenging activity; HPLC/PDA; LC-ESI/MS; Orostachys margaritifolia; nitric oxide.

Grants and funding

This research was supported by a grant from the Korea National Arboretum, “Study on the Development of Propagation and Seedling cultivation Techniques of Plants native to Korea (Funding No.: KNA1-2-40, 21-3)”, Pocheon, Republic of Korea.