The effects of high-intensity interval training and Iranian propolis extract on serum levels of TRPV4 and CYP2E1 proteins in patients with nonalcoholic fatty liver

Tunis Med. 2024 Jan 5;102(1):19-25. doi: 10.62438/tunismed.v102i1.4669.

Abstract

Introduction: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver failure, fibrosis, cirrhosis, and liver cancer, which can eventually lead to death.

Aim: To investigate the effects of high-intensity interval training (HIIT) and iranian propolis extract on serum levels of transient receptor potential cation channel subfamily V member 4 (TRPV4) and cytochrome P450 2E1 (CYP2E1) proteins in patients with NAFLD.

Methods: Thirty-two patients with NAFLD (mean±standard deviation of age: 45.1±3.6 years; body mass index: 30.0±3.6 kg/m2) were assigned in a randomized control trial to one of the following groups: HIIT (n=8), propolis supplement (n=8), propolis + HIIT (n=8), and controls (n=8). The subjects participated in eight weeks of HIIT (one bout of 1-min intervals at 80-95% of the maximal heart-rate, interspersed by two min at 50-55% of the reserve heart-rate). The Propolis supplement was taken three times a day by the patients in the form of 50 mg tablet after the main meals. Body composition, liver injury test (eg; Alanine- and Aspartate- aminotransferase levels), liver ultrasound and serum levels of TRPV4 and CYP2E1 were measured before and after intervention. One-way analysis of variance was used to compare post-tests among the groups.

Results: HIIT significantly reduced serum levels of TRPV4 protein (p=0.001). The reduction in CYP2E1 was not significant in HIIT group (p=0.075). Propolis consumption had no significant effect on serum levels of CYP2E1 protein (p=0.059), and TRPV4 (p=0.072). There was a significant decrease in TRPV4 and CYP2E1 in the HIIT (p=0.001) and propolis supplement (p=0.032) groups.

Conclusion: HIIT and propolis supplementation can be used to reduce TRPV4 and CYP2E1, which in turn reduces oxidative stress and inflammation in patients with NAFLD.

Keywords: ALT; AST; Liver enzymes; Oxidative stress; Physical activity; Sports; Visceral fat.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Cytochrome P-450 CYP2E1 / metabolism
  • Cytochrome P-450 CYP2E1 / pharmacology
  • Fibrosis
  • High-Intensity Interval Training*
  • Humans
  • Iran
  • Liver / pathology
  • Middle Aged
  • Non-alcoholic Fatty Liver Disease* / therapy
  • Propolis* / metabolism
  • Propolis* / pharmacology
  • TRPV Cation Channels / metabolism
  • TRPV Cation Channels / pharmacology

Substances

  • Cytochrome P-450 CYP2E1
  • Propolis
  • TRPV Cation Channels
  • TRPV4 protein, human