Generation of human-induced pluripotent stem cell line from PBMC of healthy donor using integration-free Sendai virus technology

Jaganmay Sarkar; Shreya Dhepe; Amrita Shivalkar; Rutuja Kuhikar; Shruti More; Vijay Bhaskar Reddy Konala; Paresh Bhanushali; Amit Khanna

doi:10.1016/j.scr.2024.103402

Generation of human-induced pluripotent stem cell line from PBMC of healthy donor using integration-free Sendai virus technology

Stem Cell Res. 2024 Mar 24:77:103402. doi: 10.1016/j.scr.2024.103402. Online ahead of print.

Authors

Jaganmay Sarkar¹, Shreya Dhepe¹, Amrita Shivalkar¹, Rutuja Kuhikar¹, Shruti More¹, Vijay Bhaskar Reddy Konala¹, Paresh Bhanushali¹, Amit Khanna²

Affiliations

¹ Department of Integrated Drug Discovery and Development, C113 Yashraj Biotechnology Ltd, TTC Industrial Area MIDC, Pawnae, Navi Mumbai 400705, Maharastra, India.
² Department of Integrated Drug Discovery and Development, C113 Yashraj Biotechnology Ltd, TTC Industrial Area MIDC, Pawnae, Navi Mumbai 400705, Maharastra, India. Electronic address: amit.khanna@yashraj.com.

PMID: 38547666
DOI: 10.1016/j.scr.2024.103402

Abstract

We developed a well-characterized human induced pluripotent stem cell (iPSC) line obtained from healthy individuals' peripheral blood mononuclear cells (PBMC). The PBMCs were primed and reprogrammed using a non-integrating sendai viral vector, and the iPSC lines demonstrated complete differentiation capacity. This line, YBLi004-A, is available and registered in the human pluripotent stem cell registry. The line's legitimacy was validated using pluripotent marker expression, in vitro differentiation into three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis. This iPSC line could be used as a healthy control for studies involving disease-specific-iPSCs, e.g. drug toxicity and efficacy testing.