Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1985 Sep;5(9):2389-98.
doi: 10.1128/mcb.5.9.2389.

The Two Alpha-Tubulin Genes of Chlamydomonas Reinhardi Code for Slightly Different Proteins

Free PMC article
Comparative Study

The Two Alpha-Tubulin Genes of Chlamydomonas Reinhardi Code for Slightly Different Proteins

C D Silflow et al. Mol Cell Biol. .
Free PMC article

Abstract

Full-length cDNA clones corresponding to the transcripts of the two alpha-tubulin genes in Chlamydomonas reinhardi were isolated. DNA sequence analysis of the cDNA clones and cloned gene fragments showed that each gene contains 1,356 base pairs of coding sequence, predicting alpha-tubulin products of 451 amino acids. Of the 27 nucleotide differences between the two genes, only two result in predicted amino acid differences between the two gene products. In the more divergent alpha 2 gene, a leucine replaces an arginine at amino acid 308, and a valine replaces a glycine at amino acid 366. The results predicted that two alpha-tubulin proteins with different net charges are produced as primary gene products. The predicted amino acid sequences are 86 and 70% homologous with alpha-tubulins from rat brain and Schizosaccharomyces pombe, respectively. Each gene had two intervening sequences, located at identical positions. Portions of an intervening sequence highly conserved between the two beta-tubulin genes are also found in the second intervening sequence of each of the alpha genes. These results, together with our earlier report of the beta-tubulin sequences in C. reinhardi, present a picture of the total complement of genetic information for tubulin in this organism.

Similar articles

See all similar articles

Cited by 90 articles

See all "Cited by" articles

References

    1. J Cell Biol. 1967 Jun;33(3):543-71 - PubMed
    1. Mol Cell Biol. 1984 Mar;4(3):424-34 - PubMed
    1. J Cell Biol. 1968 Aug;38(2):403-25 - PubMed
    1. Science. 1977 Apr 8;196(4286):180-2 - PubMed
    1. J Biol Chem. 1978 Apr 10;253(7):2483-95 - PubMed

Publication types

Associated data

LinkOut - more resources

Feedback