Algicidal activity synchronized with nitrogen removal by actinomycetes: Algicidal mechanism, stress response of algal cells, denitrification performance, and indigenous bacterial community co-occurrence

Ben Ma; Anyi Li; Shengnan Chen; Honghong Guo; Nan Li; Sixuan Pan; Kaige Chen; Hanyan Liu; Dmitry B Kosolapov; Xiang Liu; Wei Zhi; Zhongbing Chen; Yuanyuan Mo; Raju Sekar; Tinglin Huang; Haihan Zhang

doi:10.1016/j.jhazmat.2024.134117

Algicidal activity synchronized with nitrogen removal by actinomycetes: Algicidal mechanism, stress response of algal cells, denitrification performance, and indigenous bacterial community co-occurrence

J Hazard Mater. 2024 May 15:470:134117. doi: 10.1016/j.jhazmat.2024.134117. Epub 2024 Mar 24.

Authors

Ben Ma¹, Anyi Li¹, Shengnan Chen¹, Honghong Guo², Nan Li¹, Sixuan Pan¹, Kaige Chen¹, Hanyan Liu¹, Dmitry B Kosolapov³, Xiang Liu¹, Wei Zhi⁴, Zhongbing Chen⁵, Yuanyuan Mo⁶, Raju Sekar⁷, Tinglin Huang¹, Haihan Zhang⁸

Affiliations

¹ Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Provincial Field Scientific Observation and Research Station of Water Quality in Qinling Mountains, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
² Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Provincial Field Scientific Observation and Research Station of Water Quality in Qinling Mountains, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: guohh0510@xauat.edu.cn.
³ Papanin Institute for Biology of Inland Waters of Russian Academy of Sciences (IBIW RAS) 109, Borok, Nekouz, Yaroslavl 152742, Russia.
⁴ Department of Civil and Environmental Engineering, the Pennsylvania State University, USA.
⁵ Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, Praha-Suchdol, 16500, Czech Republic.
⁶ Aquatic EcoHealth Group, Key Laboratory of Urban Environment and Health, Fujian Key Laboratory of Watershed Ecology, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
⁷ Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou 215123, China.
⁸ Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Provincial Field Scientific Observation and Research Station of Water Quality in Qinling Mountains, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: zhanghaihan@xauat.edu.cn.

PMID: 38554519
DOI: 10.1016/j.jhazmat.2024.134117

Abstract

The harmful algal blooms (HABs) can damage the ecological equilibrium of aquatic ecosystems and threaten human health. The bio-degradation of algal by algicidal bacteria is an environmentally friendly and economical approach to control HABs. This study applied an aerobic denitrification synchronization algicidal strain Streptomyces sp. LJH-12-1 (L1) to control HABs. The cell-free filtrate of the strain L1 showed a great algolytic effect on bloom-forming cyanobacterium, Microcystis aeruginosa (M. aeruginosa). The optimal algicidal property of strain L1 was indirect light-dependent algicidal with an algicidal rate of 85.0%. The functional metabolism, light-trapping, light-transfer efficiency, the content of pigments, and inhibition of photosynthesis of M. aeruginosa decreased after the addition of the supernatant of the strain L1 due to oxidative stress. Moreover, 96.05% nitrate removal rate synchronized with algicidal activity was achieved with the strain L1. The relative abundance of N cycling functional genes significantly increased during the strain L1 effect on M. aeruginosa. The algicidal efficiency of the strain L1 in the raw water was 76.70% with nitrate removal efficiency of 81.4%. Overall, this study provides a novel route to apply bacterial strain with the property of denitrification coupled with algicidal activity in treating micro-polluted water bodies.

Keywords: Algicidal bacteria; Bacterial and algal communities; Functional gene; Harmful algal blooms; Photosynthetic system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Denitrification*
Harmful Algal Bloom*
Microcystis* / metabolism
Nitrates / metabolism
Nitrogen / metabolism
Photosynthesis
Streptomyces / metabolism

Substances

Nitrogen
Nitrates