Plant-Based Decellularization: A Novel Approach for Perfusion-Compatible Tissue Engineering Structures

J Microbiol Biotechnol. 2024 May 28;34(5):1003-1016. doi: 10.4014/jmb.2401.01024. Epub 2024 Feb 29.


This study explores the potential of plant-based decellularization in regenerative medicine, a pivotal development in tissue engineering focusing on scaffold development, modification, and vascularization. Plant decellularization involves removing cellular components from plant structures, offering an eco-friendly and cost-effective alternative to traditional scaffold materials. The use of plant-derived polymers is critical, presenting both benefits and challenges, notably in mechanical properties. Integration of plant vascular networks represents a significant bioengineering breakthrough, aligning with natural design principles. The paper provides an in-depth analysis of development protocols, scaffold fabrication considerations, and illustrative case studies showcasing plant-based decellularization applications. This technique is transformative, offering sustainable scaffold design solutions with readily available plant materials capable of forming perfusable structures. Ongoing research aims to refine protocols, assess long-term implications, and adapt the process for clinical use, indicating a path toward widespread adoption. Plant-based decellularization holds promise for regenerative medicine, bridging biological sciences with engineering through eco-friendly approaches. Future perspectives include protocol optimization, understanding long-term impacts, clinical scalability, addressing mechanical limitations, fostering collaboration, exploring new research areas, and enhancing education. Collectively, these efforts envision a regenerative future where nature and scientific innovation converge to create sustainable solutions, offering hope for generations to come.

Keywords: Green tissue engineering; plant scaffolds; regenerative innovation; sustainable bioengineering; vascularized structures.

Publication types

  • Review

MeSH terms

  • Decellularized Extracellular Matrix / chemistry
  • Extracellular Matrix / chemistry
  • Humans
  • Perfusion / methods
  • Plants
  • Regenerative Medicine* / methods
  • Tissue Engineering* / methods
  • Tissue Scaffolds* / chemistry


  • Decellularized Extracellular Matrix