1-Aminooxy-3-aminopropane, a new and potent inhibitor of polyamine biosynthesis that inhibits ornithine decarboxylase, adenosylmethionine decarboxylase and spermidine synthase

Biochem Biophys Res Commun. 1985 Jul 31;130(2):596-602. doi: 10.1016/0006-291x(85)90458-9.


1-Aminooxy-3-aminopropane was shown to be a potent competitive inhibitor (Ki = 3.2 nM) of homogenous mouse kidney ornithine decarboxylase, a potent irreversible inhibitor (Ki = 50 microM) of homogeneous liver adenosylmethionine decarboxylase and a potent competitive (Ki = 2.3 microM) of homogeneous bovine brain spermidine synthase. It did not inhibit homogeneous bovine brain spermine synthase and it did not serve as a substrate for spermidine synthase. The compound did not inhibit tyrosine aminotransferase, alanine aminotransferase or aspartate aminotransferase, which are pyridoxal phosphate-containing enzymes like ornithine decarboxylase. The inactivation of adenosylmethionine decarboxylase was partially prevented by pyruvate, which is the coenzyme of adenosylmethionine decarboxylase, and by the substrate, adenosylmethionine. 1-Aminooxy-3-aminopropane at 0.5 mM concentration inhibited the growth of HL-60 promyelocytic leukemia cells and this inhibition was prevented by spermidine but not by putrescine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosylmethionine Decarboxylase / antagonists & inhibitors
  • Animals
  • Brain / enzymology
  • Cattle
  • Humans
  • Kidney / enzymology
  • Leukemia, Myeloid, Acute / enzymology
  • Mice
  • Ornithine Decarboxylase Inhibitors
  • Polyamines / biosynthesis*
  • Propylamines / pharmacology*
  • Putrescine / pharmacology
  • Rats
  • Spermidine / pharmacology
  • Spermidine Synthase / antagonists & inhibitors


  • Ornithine Decarboxylase Inhibitors
  • Polyamines
  • Propylamines
  • 1-aminooxy-3-aminopropane
  • Spermidine Synthase
  • Adenosylmethionine Decarboxylase
  • Spermidine
  • Putrescine