Insight into the structural characteristics of self-assembled liposome with epigallocatechin gallate/alcohol dehydrogenase

Xiaodan Zhang; Lili Liu; Xueyuan Jiao; Kenan Su; Weiwei Cheng; Baocheng Xu

doi:10.1016/j.colsurfb.2024.113917

Insight into the structural characteristics of self-assembled liposome with epigallocatechin gallate/alcohol dehydrogenase

Colloids Surf B Biointerfaces. 2024 Apr 12:238:113917. doi: 10.1016/j.colsurfb.2024.113917. Online ahead of print.

Authors

Xiaodan Zhang¹, Lili Liu², Xueyuan Jiao¹, Kenan Su¹, Weiwei Cheng¹, Baocheng Xu¹

Affiliations

¹ College of Food and Bioengineering, National Experimental Teaching Demonstration Center for Food Processing and Security, Henan Engineering Technology Research Center of Food Raw Materials, International Joint Laboratory of Food Processing and Quality Safety Control of Henan Province, Henan Engineering Technology Research Center of Food Microbiology, Henan University of Science and Technology, Luoyang 471023, PR China.
² College of Food and Bioengineering, National Experimental Teaching Demonstration Center for Food Processing and Security, Henan Engineering Technology Research Center of Food Raw Materials, International Joint Laboratory of Food Processing and Quality Safety Control of Henan Province, Henan Engineering Technology Research Center of Food Microbiology, Henan University of Science and Technology, Luoyang 471023, PR China. Electronic address: yangliuyilang@126.com.

PMID: 38615391
DOI: 10.1016/j.colsurfb.2024.113917

Abstract

In this study, the encapsulation and structural characteristics of the self-assembled liposome formed by epigallocatechin gallate (EGCG) and alcohol dehydrogenase (ADH) were studied. According to the results, EGCG significantly increased the catalytic activity of ADH with a 33.33 % activation rate and the liposomes were able to entrap EGCG-ADH with an effectiveness of 88.94 %. The self-assembled monolayers had nanometer-sized particles, and the excellent self-assembled system was demonstrated by the low PDI value and high surface absolute potential. The scanning electron microscope showed that the self-assembled liposome was honeycomb, groove-shaped, and rough. The spectroscopic results showed that EGCG-ADH complex was formed through hydrogen bond, which changed the secondary structure of the liposome, and verified EGCG-ADH liposome system was successfully prepared. In vitro digestion experiments showed that the gastrointestinal tolerance and antioxidant activity of EGCG-ADH liposomes were significantly higher than those of free EGCG-ADH.

Keywords: Alcohol dehydrogenase; Epigallocatechin gallate; Liposomes; Self-assembly; Structural characteristics.