Quantitative measurement of acute myocardial infarction cardiac biomarkers by "All-in-One" immune microfluidic chip for early diagnosis of myocardial infarction

Zhaokui Zeng; Huimin Li; Qi Li; Ruowei Sun; Xun Zhang; Di Zhang; Qubo Zhu; Chuanpin Chen

doi:10.1016/j.saa.2024.124256

Quantitative measurement of acute myocardial infarction cardiac biomarkers by "All-in-One" immune microfluidic chip for early diagnosis of myocardial infarction

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Apr 10:315:124256. doi: 10.1016/j.saa.2024.124256. Online ahead of print.

Authors

Zhaokui Zeng¹, Huimin Li², Qi Li¹, Ruowei Sun³, Xun Zhang³, Di Zhang⁴, Qubo Zhu¹, Chuanpin Chen⁵

Affiliations

¹ Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
² Yueyang Inspection and Testing Center, Yueyang 414000, China.
³ Hunan Zaochen Nanorobot Co., Ltd, Liuyang 410300, China.
⁴ Department of Laboratory, The Third Xiangya Hospital, Central South University, Changsha 410013, China.
⁵ Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China. Electronic address: Chuanpin.chen@csu.edu.cn.

PMID: 38615418
DOI: 10.1016/j.saa.2024.124256

Abstract

Acute myocardial infarction (AMI) is a life-threatening condition with a narrow treatment window, necessitating rapid and accurate diagnostic methods. We present an "all-in-one" convenient and rapid immunoassay system that combines microfluidic technology with a colloidal gold immunoassay. A degassing-driven chip replaces a bulky external pump, resulting in a user-friendly and easy-to-operate immunoassay system. The chip comprises four units: an inlet reservoir, an immunoreaction channel, a waste pool, and an immunocomplex collection chamber, allowing single-channel flow for rapid and accurate AMI biomarker detection. In this study, we focused on cardiac troponin I (cTnI). With a minimal sample of just 4 μL and a total detection time of under 3 min, the chip enabled a quantitative visual analysis of cTnI concentration within a range of 0.5 ∼ 60.0 ng mL^-1. This all-in-one integrated microfluidic chip with colloidal gold immunoassay offers a promising solution for rapid AMI diagnosis. The system's portability, small sample requirement, and quantitative visual detection capabilities make it a valuable tool for AMI diagnostics.

Keywords: AMI cardiac biomarkers Myocardial infarction; All-in-One; Immune microfluidic chip; Quantitative analysis.

Publication types

Review