Study on the pharmacokinetics, tissue distribution and excretion of Penthorum chinense Pursh in normal and acute alcoholic liver injury rats using validated UPLC-MS/MS method

Haitao Tian; Miao Hou; Xinyi Zhu; Chunying Cai; Pan Zhao; Ying Yang; Chunguo Yang; Zhipeng Deng

doi:10.1016/j.jpba.2024.116157

Study on the pharmacokinetics, tissue distribution and excretion of Penthorum chinense Pursh in normal and acute alcoholic liver injury rats using validated UPLC-MS/MS method

J Pharm Biomed Anal. 2024 Apr 16:245:116157. doi: 10.1016/j.jpba.2024.116157. Online ahead of print.

Authors

Haitao Tian¹, Miao Hou¹, Xinyi Zhu¹, Chunying Cai¹, Pan Zhao¹, Ying Yang², Chunguo Yang³, Zhipeng Deng⁴

Affiliations

¹ College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
² Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
³ Shandong Yifang Pharmaceutical Co., Ltd, Linyi 253000, China.
⁴ College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. Electronic address: dengzhipeng@sdutcm.edu.cn.

PMID: 38636192
DOI: 10.1016/j.jpba.2024.116157

Abstract

Penthorum chinense Pursh (PCP), as a traditional medicine of Miao nationality in China, is often used for the treatment of various liver diseases. At present, information regarding the in vivo process of PCP is lacking. Herein, a sensitive and robust ultra-performance liquid chromatography tandem with mass spectrometry (UPLC-MS/MS) was developed and validated for the quantification of several components to study their pharmacokinetics, tissues distribution and excretion in normal and acute alcoholic liver injury (ALI) rats. Prepared samples were separated on a Thermo C₁₈ column (4.6 mm × 50 mm, 2.4 μm) using water containing 0.1 % formic acid (A) and acetonitrile (B) as the mobile phase for gradient elution. Negative electrospray ionization was performed using multiple reaction monitoring (MRM) mode for each component. The validated UPLC-MS/MS assay gave good linearity, accuracy, precision, recovery rate, matrix effect and stability. This method was successfully applied to the pharmacokinetics, tissue distribution and excretion in normal and acute ALI rats. There were differences in pharmacokinetic process, tissue distribution and excretion characteristics, indicating that ALI had a significant influence on the in vivo process of PCP in rats. The research provided an experimental basis for the study of PCP quality control and further application in the clinic.

Keywords: Penthorum chinense Pursh; Pharmacokinetics; Tissue distribution; UPLC-MS/MS.