Analysis of HLA-DQ molecules with a monoclonal antibody detecting a DQ polymorphism absent from DQW1 homozygous cells

Hum Immunol. 1985 Nov;14(3):206-19. doi: 10.1016/0198-8859(85)90229-0.


We have produced a monoclonal antibody detecting an HLA class II determinant absent from DR1,2,W6, and W10 (DQW1) homozygous B lymphoblastoid cell lines (BLCL). The antibody, SFR16-PI.2, immunoprecipitates molecules with electrophoretic mobilities of DQ (DS/DC) molecules from DR7 homozygous cell lines. SFR16-PI.2 binds more sets of class II molecules from internally labeled DR7 homozygous cell membranes than from externally labeled extracts from the same cell line. Depletion of DR molecules from internally-labeled membranes indicates that SFR16-PI.2, in addition to reacting with DQ molecules, also reacts with an epitope on a biosynthetic intermediate of DR molecules, which is lost on the mature DR molecule. SFR16-PI.2-reactive molecules were examined on a DR5 homozygous cell line, where they could be compared to those isolated by two other monoclonal antibodies with DQ specificity, namely IVD12 and Leu-10. All three monoclonal antibodies isolated two sets of DQ molecules with varying degrees of affinity for one of the molecules. The serological and biochemical data presented suggest that SFR16-PI.2 detects the alternate alleles of the locus encoding DQW1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Antibodies, Monoclonal*
  • Cells, Cultured
  • Chemical Precipitation
  • HLA-DQ Antigens
  • Heterozygote
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Isoelectric Point
  • Molecular Weight
  • Polymorphism, Genetic


  • Antibodies, Monoclonal
  • HLA-DQ Antigens
  • Histocompatibility Antigens Class II