Withanicandrin Isolated from Datura Ferox Promotes Antinociception by Modulating the Asics and TRPS Channels and Anti-Inflammation in Adult Zebrafish

Chem Biodivers. 2024 Jul;21(7):e202400538. doi: 10.1002/cbdv.202400538. Epub 2024 Jun 7.

Abstract

This is the first study to analyze the anti-inflammatory and antinociceptive effect of withanicandrin, isolated from Datura Ferox leaves, and the possible mechanism of action involved in adult zebrafish (ZFa). To this end, the animals were treated intraperitoneally (i. p.) with withanicandrin (4; 20 and 40 mg/kg; 20 μL) and subjected to locomotor activity and acute toxicity. Nociception tests were also carried out with chemical agents, in addition to tests to evaluate inflammatory processes induced by κ-Carrageenan 1.5 % and a Molecular Docking study. As a result, withanicandrin reduced nociceptive behavior by capsaicin at a dose of 40 mg/kg and by acid saline at doses of 4 and 40 mg/kg, through neuromodulation of TRPV1 channels and ASICs, identified through blocking the antinociceptive effect of withanicandrin by the antagonists capsazepine and naloxone. Furthermore, withanicandrin caused an anti-inflammatory effect through the reduction of abdominal edema, absence of leukocyte infiltrate in the liver tissue and reduction of ROS in thel liver tissue and presented better affinity energy compared to control morphine (TRPV1) and ibuprofen (COX-1 and COX-2).

Keywords: ASICs channels; Analgesia; Inflammatory; TRPV1 channel; Vitanolides.

MeSH terms

  • Acid Sensing Ion Channels / metabolism
  • Analgesics* / chemistry
  • Analgesics* / isolation & purification
  • Analgesics* / pharmacology
  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology
  • Carrageenan
  • Dose-Response Relationship, Drug
  • Edema / chemically induced
  • Edema / drug therapy
  • Molecular Docking Simulation
  • Molecular Structure
  • Plant Leaves / chemistry
  • Structure-Activity Relationship
  • TRPV Cation Channels / antagonists & inhibitors
  • TRPV Cation Channels / metabolism
  • Zebrafish*

Substances

  • Analgesics
  • Acid Sensing Ion Channels
  • Anti-Inflammatory Agents
  • Carrageenan
  • TRPV Cation Channels