Preparation of a β-cyclodextrin grafted magnetic biochar for efficient extraction of four antiepileptic drugs in plasma samples

Qing Wang; Xinyu Shi; Shao-Feng Tang; Huanhuan Wang; Yuan Chen; Na Zhang

doi:10.1016/j.chroma.2024.464893

Preparation of a β-cyclodextrin grafted magnetic biochar for efficient extraction of four antiepileptic drugs in plasma samples

J Chromatogr A. 2024 Apr 11:1724:464893. doi: 10.1016/j.chroma.2024.464893. Online ahead of print.

Authors

Qing Wang¹, Xinyu Shi², Shao-Feng Tang², Huanhuan Wang³, Yuan Chen², Na Zhang²

Affiliations

¹ Institute of Collaborative Innovation in Great Health, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China. Electronic address: qingwang819@tjcu.edu.cn.
² Institute of Collaborative Innovation in Great Health, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China.
³ Department of stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.

PMID: 38643615
DOI: 10.1016/j.chroma.2024.464893

Abstract

Simultaneous monitoring of plasma concentration levels of multiple antiepileptic drugs (AEDs) is essential for dose adjustment in comprehensive epilepsy treatment, necessitating a sensitive technique for accurate extraction and determination of AEDs. Herein, a magnetic solid-phase extraction (MSPE) technique on the basis of modified biochar (BC) is investigated to extract four AEDs from plasma, in conjunction with high performance liquid chromatography. BC derived from Zizyphus jujuba seed shells was activated by phosphoric acid (PBC) and magnetized via coprecipitation to produce MPBC. The MPBCCD obtained after modification with β-cyclodextrin (CD) was characterized and evaluated for adsorption. It exhibited fast adsorption kinetics based on second-order kinetics and satisfactory adsorption capacity for AEDs. Then it was employed as the MSPE adsorbent and the influencing parameters were optimized. The enrichment factor was 18.75. The validation analysis revealed a favorable linearity that ranged from 0.04 to 20 μg·mL^-1 along with a low limit of detection of 6.85 to 10.19 ng·mL^-1. The recovery of the AEDs ranged from 78.7 to 109.2 %, with relative standard deviations below 6.7 %. Using quantum chemistry theory calculations and experimental results analysis, the adsorption mechanism was investigated. It disclosed that the suggested strategy built upon MPBCCD was appropriate for the assessment of AEDs in plasma and expanded the usage of BC as the environmentally favorable matrix for the analysis of biological samples.

Keywords: Antiepileptic drugs; Biochar; High performance liquid chromatography; Magnetic solid-phase extraction; β-Cyclodextrin.